This research examined passengers' satisfaction with the Lagos-Ibadan rail transport service. The study examined the socio-economic characteristics of the passengers, investigated the purpose of trip, and assessed users’ satisfaction with railway services to and from Lagos-Ibadan. Data for this study were generated through an online survey administered between March and May 2024. Data obtained were analyzed using descriptive statistics which consist of simple frequency and percentage and Summation of Weight Value (SWV). Findings revealed that Users' are satisfied with attributes related to comfort such as temperature, seating capacity, quality of train, cleanliness, level of noise pollution, and waiting conditions with RMI between 3.1 and 3.9. However, attributes that relate to travel time such as access time, waiting time, transfer time, and punctuality all have RMI that is below 3.0000. The study, therefore recommended that attributes that relate to travel time such as access time, waiting time, transfer time, and punctuality should be improved upon. Also, the management of the Lagos-Ibadan rail service should frequently conduct rail user survey to assess their overall satisfaction with their most recent trip.