Different people from different socio-economic levels and hygiene status use the ATMs daily and increase the chances of hand-borne transmission of microorganisms to the machines’ surfaces. A total of 72 colonies and 56 colonies of four fungi belonging to three genera were repeatedly isolated from the surfaces of the banks’ ATMs’ touch-screens and keyboards. They are; Fusarium moniliforme, Malassezia globosa, Phanaerochate carnosa and Fusarium oxysporum. A total of 217 colonies and 158 colonies of three bacteria belonging to two genera were repeatedly isolated and they were identified based on standard tests for the identification of bacteria. They include; Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis. The microbial load of the banks’ ATMs’ touch-screens is fairly higher than that of the banks’ ATMs’ keyboards. UBA bank recorded the highest microbial load. All the selected banks’ ATMs were contaminated with varied fungi and bacteria, though with disparity. The public should be sensitized to avoid direct hand-to-mouth contact after an ATM use to avoid potential inadvertent inoculation by the users.